圣路易斯华盛顿大学

腾讯会议：131-712-443

报告摘要：

Cilia are hair-like organelles protrude from the surface of most eukaryotic cells and are responsible for cellular motility, fluid flow and sensory perception. The axoneme of most motile cilia consists of nine doublet microtubules (DMT) arranged in a circle around a central pair of singlet MTs, generating a 9+2 arrangement. A large T-shaped protein complex named radial spoke connects the doublet MTs to the central apparatus. In our recent work, we used single-particle cryo-EM to visualize and build atomic models of the repeating structures of several native axonemal complexes (DMT, radial spoke and central apparatus), which reveals the identities, positions, repeat lengths, and interactions of more than 120 associated proteins including microtubule inner proteins (MIPs). These structures demonstrate how these proteins establish the unique architecture of DMTs and maintain coherent periodicities along the axoneme. Our work provides a molecular atlas for interpreting genetic, biochemical, and physiological data from different cell types and for understanding the etiology of human ciliopathies.

张锐简历：

张锐，圣路易斯华盛顿大学医学院生物化学与分子生物物理学系，助理教授。2005年本科毕业于南京大学，获生物化学学士学位。后赴美攻读博士，在贝勒医学院师从Wah Chiu教授，2010年获结构生物学（Cryo-EM）博士学位。2010年至2015年，以博士后身份在美国劳伦斯伯克利国家实验室Eva Nogales教授课题组从事微管的冷冻电镜结构解析。已在Cell，Nature Structural & Molecular Biology，Nature Communications等国际著名杂志上发表多篇SCI论文。实验室主要利用高分辨单颗粒冷冻电镜来研究从绿藻到哺乳动物等各种生物体中直接分离出大分子复合物的原位结构，目前大部分项目与微管和纤毛有关。

邀请人：丁玮（82649477）