邀请人：叶方富 研究员（fye@iphy.ac.cn）

腾讯会议号：709-885-797

报告摘要：

Cryo-electron ptychography is an emerging cryoEM imaging method developed in recent years. Originating from ptychography in 4D-STEM, this technique has achieved significant progress in materials science, breaking the resolution limits of conventional transmission electron microscopy. However, in life science applications, due to the radiation-sensitive nature of biological specimens, data acquisition requires extremely low dose, which has long constrained the resolution of this computational imaging-based ptychographic approach. Through systematic investigation of key technical factors affecting cryo-ptychography resolution, powered by the electron counting, we have made important progress by elevating the resolution of ptychographic single-particle analysis from nanometer scale to near-atomic resolution - reaching practical utility levels. Our study also revealed multiple advantages of cryo-electron ptychography for biological specimen imaging. These findings demonstrate that cryo-electron ptychography holds promise to become the core imaging principle for next-generation cryoEM systems.

报告人简介：

Xueming Li is currently a professor at the School of Life Science, Tsinghua University, and principal investigator of the Beijing Advanced Innovation Center for Structural Biology and Tsinghua-Peking Joint Center for Life Sciences. He received his Ph.D in condensed matter physics from the Institute of Physics at the Chinese Academy of Sciences in 2009. Then, he joined Yifan Cheng's laboratory at the University of California San Francisco as a postdoctoral scholar. Xueming Li has been at Tsinghua University since 2014. Xueming Li has been engaged in research on electron cryo-microscopy (cryoEM) methods and techniques, as well as the applications of cryoEM in structural biology. Currently, Xueming Li's research focuses on the methodological and technological development of cryo-electron tomography (cryoET) with the goal of obtaining in situ structures of cellular organelles and biological macromolecules, and exploring new technologies for the next generation of cryoEM.